Louis Pasteur
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Louis Pasteur
VI. Anthrax Research

Pasteur also determined the natural history of anthrax, a fatal disease of cattle. He proved that anthrax is caused by a particular bacillus and suggested that animals could be given anthrax in a mild form by vaccinating them with attenuated (weakened) bacilli, thus providing immunity from potentially fatal attacks. In order to prove his theory, Pasteur began by inoculating 25 sheep; a few days later he inoculated these and 25 more sheep with an especially strong inoculant, and he left 10 sheep untreated. He predicted that the second 25 sheep would all perish and concluded the experiment dramatically by showing, to a skeptical crowd, the carcasses of the 25 sheep lying side by side.